FlowCal’s Python API Tutorial

FlowCal is, at its core, a Python library that a programmer can use to analyze flow cytometry data in a more flexible way than with the Excel UI. This section gives an overview of the abilities of FlowCal from a programmer’s perspective. The tutorials below are listed below in order of increasing complexity. We recommend the reader to go through them in order.

Note

The FlowCal Python API tutorial assumes that the reader is familiar with Python, numpy and matplotlib. It also assumes that the reader has Python 2.7 installed, as well as FlowCal and all its dependencies. For more information on installation, refer to the Getting started section.

• Reading Flow Cytometry Data
• Transforming Flow Cytometry Data
• Plotting Flow Cytometry Data
• Gating Flow Cytometry Data
• Calibrating Flow Cytometry Data to MEF
• Processing FCS Files with the Excel UI