How to use FlowCal’s Excel UI

To use the FlowCal’s Excel UI, follow these steps:

1. Make and save an Excel file indicating the FCS files to process. Click here for information on how to make a properly formatted input Excel file.
2. Launch FlowCal’s Excel UI by double clicking on Run FlowCal (Windows).bat or Run FlowCal (OSX).
3. A window will appear requesting an input Excel file. Locate the Excel file made in step 1 and click on “Open”.
4. FlowCal will start processing the indicated calibration beads and cell samples. A terminal window will appear indicating the progress of the analysis.
5. When the analysis finishes, the message “Press Enter to finish...” will appear. Press Enter and close the terminal window. A set of plots and an Excel file with statistics will appear in the same directory in which the input Excel file was located.